I am trying to determine the best cut off value for a single diagnostic test by performing the ROC curve analysis. The diagnostic test has AUC of 0.99, so the test has a high discriminating potential, which is great, and I found the best cut off value by choosing the optimal cut off value with sens and spec that suits well to his intended clinical utility. However, I got a question from my peer how come that I don't have different AUC values for different cut off points, showing me the right cut off value. Based on my understanding, there cannot be different AUC for different cut off values for a single diagnostic test - am I right ? If there is a way to calculate the AUC in different cut off values, could you please share with me some recommended papers/ any literature? Thanks!